PTPN14 Monoklonaler Antikörper

PTPN14 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B8C8

Kat-Nr. : 67744-1-PBS

Synonyme

1B8C8, EC:3.1.3.48, PEZ, Protein-tyrosine phosphatase pez, PTP 36

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  WB analysis using 67744-1-Ig (same clone as 67744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67744-1-Ig (same clone as 67744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67744-1-Ig (same clone as 67744-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTPN14 antibody (67744-1-Ig, Clone: 1B8C8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67744-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67744-1-PBS bindet in WB, IHC, IF/ICC, ELISA PTPN14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTPN14 fusion protein Ag12679
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 14
• Berechnetes Molekulargewicht: 1187 aa, 135 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC101754
• Gene symbol: PTPN14
• Gene ID (NCBI): 5784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).