PTPN2 Polyklonaler Antikörper

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• KD/KO Validated

PTPN2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11214-1-AP

Synonyme

PTPN2, PTPT, TC PTP, TCELLPTP, TCPTP

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Galerie der Validierungsdaten

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  WB analysis of mouse thymus using 11214-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11214-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11214-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11214-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11214-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11214-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 11214-1-AP

  IP Result of anti-PTPN2 (IP:11214-1-AP, 4ug; Detection:11214-1-AP 1:500) with HL-60 cells lysate 4000ug.

• 

  IHC staining of human colon cancer using 11214-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11214-1-AP (PTPN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11214-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 11214-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, HL-60-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

11214-1-AP bindet in WB, IP, IHC, IF, ELISA PTPN2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN2 fusion protein Ag1709
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC016727
• Gene symbol: PTPN2
• Gene ID (NCBI): 5771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

Publikationen

Species	Application	Title
mouse	IHC	Oncogene; Overexpression of TC-PTP in murine epidermis attenuates skin tumor formation.; Authors - Mihwa Kim
human,mouse	WB,IHC	Int Immunopharmacol; Hypoxia disrupts the nasal epithelial barrier by inhibiting PTPN2 in chronic rhinosinusitis with nasal polyps; Authors - Meiping Zhang ; KD Validated
mouse	IHC	Int Immunopharmacol; Oral squamous cell carcinoma-derived EVs promote tumor progression by regulating inflammatory cytokines and the IL-17A-induced signaling pathway; Authors - Ruowei Li
human	WB	Cell Death Dis; Cryptotanshinone inhibits human glioma cell proliferation in vitro and in vivo through SHP-2-dependent inhibition of STAT3 activation.; Authors - Liang Lu ; KD Validated
mouse	WB,IHC	Sci Rep; Targeted disruption of TC-PTP in the proliferative compartment augments STAT3 and AKT signaling and skin tumor development.; Authors - Hyunseung Lee ; KO Validated
human	IF	Infect Immun; An Ehrlichia chaffeensis tandem repeat protein interacts with multiple host targets involved in cell signaling, transcriptional regulation, and vesicle trafficking.; Authors - Wakeel Abdul A