PTPN2 Polyklonaler Antikörper

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• KD/KO Validated

PTPN2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11214-1-PBS

Synonyme

T-cell protein-tyrosine phosphatase, TC PTP, PTPT, PTPN 2, PTN2

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  WB analysis of mouse thymus using 11214-1-AP (same clone as 11214-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 11214-1-AP (same clone as 11214-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11214-1-AP (same clone as 11214-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)

  IP result of anti-PTPN2 (IP:11214-1-AP, 4ug; Detection:11214-1-AP 1:500) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11214-1-AP (PTPN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 11214-1-AP (same clone as 11214-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTPN2 antibody (11214-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11214-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PTPN2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN2 fusion protein Ag1709
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC016727
• Gene symbol: PTPN2
• Gene ID (NCBI): 5771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.