PTPN2 Rekombinanter Antikörper

PTPN2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250281G2

Kat-Nr. : 85915-1-PBS

Synonyme

EC:3.1.3.48, PTN2, PTPN 2, PTPT, TC PTP

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  WB analysis using 85915-1-RR (same clone as 85915-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85915-1-RR (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85915-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02187-2

  Sandwich ELISA standard curve of MP02187-2, Human PTPN2 Recombinant Matched Antibody Pair - PBS only. 85915-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1709. 85915-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  Sample test of MP02187-2

  The mean PTPN2 concentration was determined to be 102.95 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 169.33 ng/mL in HepG2 cell extract based on a 0.90 mg/mL extract load.

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  IF Staining of HeLa using 85915-1-RR (same clone as 85915-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN2 antibody (85915-1-RR, Clone: 250281G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85915-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02187-2

  Cytometric bead array standard curve of MP02187-2, PTPN2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85915-2-PBS. Detection antibody: 85915-1-PBS. Standard: Ag1709. Range: 0.938-120 ng/mL.

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  Affinity and Kinetic Characterization of 85915-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85915-1-RR against Human PTPN2 were performed. The affinity constant is 0.313 nM.

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Geprüfte Anwendungen

Produktinformation

85915-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PTPN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTPN2 fusion protein Ag1709
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC016727
• Gene symbol: PTPN2
• Gene ID (NCBI): 5771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.