PTPN2 Rekombinanter Antikörper

PTPN2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250281G2

Kat-Nr. : 85915-1-RR

Synonyme

EC:3.1.3.48, PTN2, PTPN 2, PTPT, TC PTP

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  WB analysis using 85915-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85915-1-RR (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85915-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN2 antibody (85915-1-RR, Clone: 250281G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85915-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85915-1-RR against Human PTPN2 were performed. The affinity constant is 0.313 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Maus-Thymusgewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85915-1-RR bindet in WB, IF/ICC, ELISA PTPN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTPN2 fusion protein Ag1709
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC016727
• Gene symbol: PTPN2
• Gene ID (NCBI): 5771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.