PTPN20 Polyklonaler Antikörper

PTPN20 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19510-1-AP

Synonyme

PTPN20B, Tyrosine-protein phosphatase non-receptor type 20, PTPN20A, hPTPN20, EC:3.1.3.48

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  WB analysis using 19510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19510-1-AP (PTPN20B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse tongue using 19510-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 19510-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 19510-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 19510-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 19510-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 19510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PTPN20 antibody (19510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauszungengewebe, pig tongue tissue, rat tongue tissue
• Erfolgreiche Detektion in IHC: Mauszungengewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19510-1-AP bindet in WB, IHC, IF/ICC, ELISA PTPN20 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 20B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_015605
• Gene symbol: PTPN20B
• Gene ID (NCBI): 26095
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN20B, also named as PTPN20A, belongs to the protein-tyrosine phosphatase family and Non-receptor class subfamily. PTPN20B is a tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. PTPN20B has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. PTPN20B catalyze the reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. The antibody can recognize the isoform 1,2,7,8,10,11,13,15.