PTPN7 Polyklonaler Antikörper

PTPN7 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15286-1-AP

Synonyme

BPTP 4, HEPTP, LC PTP, LPTP, PTPN7, PTPNI

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Galerie der Validierungsdaten

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  WB analysis using 15286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15286-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15286-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

15286-1-AP bindet in WB, IHC, ELISA PTPN7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPN7 fusion protein Ag7453
• Vollständiger Name: protein tyrosine phosphatase, non-receptor type 7
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC001746
• Gene symbol: PTPN7
• Gene ID (NCBI): 5778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

Publikationen

Species	Application	Title
pig	WB	Front Cell Dev Biol; piR-121380 Is Involved in Cryo-Capacitation and Regulates Post-Thawed Boar Sperm Quality Through Phosphorylation of ERK2 via Targeting PTPN7.; Authors - Yihan Wang
human	IHC	Front Genet; Comprehensive analysis of PTPN gene family revealing PTPN7 as a novel biomarker for immuno-hot tumors in breast cancer; Authors - Fengxu Wang