PTPRH Monoklonaler Antikörper

PTPRH Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C6E8

Kat-Nr. : 68635-1-Ig

Synonyme

3C6E8, R PTP H, SAP 1, SAP1

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  WB analysis of pig spleen using 68635-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 68635-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HT-29 using 68635-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using PTPRH antibody (68635-1-Ig, Clone: 3C6E8 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Milzgewebe, A431-Zellen
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68635-1-Ig bindet in WB, IF/ICC, ELISA PTPRH und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTPRH fusion protein Ag17351
• Vollständiger Name: protein tyrosine phosphatase, receptor type, H
• Berechnetes Molekulargewicht: 1115 aa, 122 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC111716
• Gene symbol: PTPRH
• Gene ID (NCBI): 5794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRH, also known as SAP-1 (Stomach Cancer-Associated Protein-Tyrosine Phosphatase-1), is a member of the protein tyrosine phosphatase (PTP) family. These enzymes regulate cellular signaling by dephosphorylating tyrosine residues on proteins, counterbalancing kinase activity (PMID: 11278335). PTPRH is classified as a receptor-type PTP, characterized by an extracellular domain, a transmembrane region, and intracellular catalytic domains. In colorectal, gastric, and hepatocellular cancers, PTPRH is often upregulated and linked to poor prognosis (PMID: 12101188). Paradoxically, it may suppress tumorigenesis in other contexts by stabilizing cell adhesion. Overexpression in certain cancers suggests utility as a diagnostic or prognostic marker. The molecular weight of PTPRH is 122 kDa, it is reported that it can form a dimer with a molecular weight of 210 kDa (PMID:15850787).