PTPRJ Polyklonaler Antikörper

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• KD/KO Validated

PTPRJ Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55123-1-PBS

Synonyme

DEP-1, DEP1, DEP 1, Density-enhanced phosphatase 1, Density enhanced phosphatase 1

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  WB analysis of multi-cells/tissue using 55123-1-AP (same clone as 55123-1-PBS)

  WB result of 55123-1-AP. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 55123-1-AP (same clone as 55123-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis using 55123-1-AP (same clone as 55123-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 55123-1-AP (same clone as 55123-1-PBS)

  WB result of PTPRJ antibody (55123-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRJ transfected HeLa cells. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 55123-1-AP (same clone as 55123-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55123-1-AP (PTPRJ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 55123-1-AP (same clone as 55123-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55123-1-AP (PTPRJ Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55123-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55123-1-PBS bindet in WB, IHC, Indirect ELISA PTPRJ und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein tyrosine phosphatase, receptor type, J
• Berechnetes Molekulargewicht: 146 kDa
• Beobachtetes Molekulargewicht: 146-170 kDa
• GenBank-Zugangsnummer: NM_002843
• Gene symbol: PTPRJ
• Gene ID (NCBI): 5795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRJ, also named as CD148 and DEP1, belongs to the protein-tyrosine phosphatase family and Receptor class 3 subfamily. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRJ may contribute to the mechanism of contact inhibition of cell growth. PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). It was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction. The antibody is specific to PTPRJ.