PTPRO Monoklonaler Antikörper

PTPRO Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F2B4

Kat-Nr. : 67000-1-PBS

Synonyme

2F2B4, EC:3.1.3.48, GLEPP1, PTP U2, PTPase U2

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  WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:3000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67000-1-Ig (PTPRO antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67000-1-Ig (same clone as 67000-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using PTPRO antibody (67000-1-Ig, Clone: 2F2B4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ACE2 antibody (21115-1-AP, green). This data was developed using the same antibody clone with 67000-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67000-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PTPRO und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTPRO fusion protein Ag8284
• Vollständiger Name: protein tyrosine phosphatase, receptor type, O
• Berechnetes Molekulargewicht: 138 kDa
• Beobachtetes Molekulargewicht: 160 kDa, 180-220 kDa
• GenBank-Zugangsnummer: BC035960
• Gene symbol: PTPRO
• Gene ID (NCBI): 5800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).