PTPRO Monoklonaler Antikörper

PTPRO Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2F2B4

Kat-Nr. : CL594-67000

Synonyme

2F2B4, EC:3.1.3.48, GLEPP1, PTP U2, PTPase U2

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  IF Staining of human kidney using CL594-67000

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 PTPRO antibody (CL594-67000, Clone: 2F2B4 ) at dilution of 1:100.

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  IF Staining of mouse small intestine using CL594-67000

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CoraLite®594 PTPRO antibody (CL594-67000, Clone: 2F2B4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Nierengewebe, Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67000 bindet in IF-P PTPRO und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTPRO fusion protein Ag8284
• Vollständiger Name: protein tyrosine phosphatase, receptor type, O
• Berechnetes Molekulargewicht: 138 kDa
• Beobachtetes Molekulargewicht: 160 kDa, 180-220 kDa
• GenBank-Zugangsnummer: BC035960
• Gene symbol: PTPRO
• Gene ID (NCBI): 5800
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).