PTPRR Polyklonaler Antikörper

PTPRR Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 17937-1-AP

Synonyme

Ch 1PTPase, EC PTP, ECPTP, NC PTPCOM1, PCPTP1, PTP SL, PTPBR7, PTPRQ, PTPRR, R PTP R

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Galerie der Validierungsdaten

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  WB analysis using 17937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17937-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17937-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17937-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, Maushirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

17937-1-AP bindet in WB, IF, ELISA PTPRR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTPRR fusion protein Ag12145
• Vollständiger Name: protein tyrosine phosphatase, receptor type, R
• Berechnetes Molekulargewicht: 657 aa, 74 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 47 kDa, 65 kDa
• GenBank-Zugangsnummer: BC110900
• Gene symbol: PTPRR
• Gene ID (NCBI): 5801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites.

Publikationen

Species	Application	Title
mouse	WB	J Affect Disord; PTPRR regulates ERK dephosphorylation in depression mice model.; Authors - Xinrong Li
human	WB	BMC Cancer; Androgen-regulation of the protein tyrosine phosphatase PTPRR activates ERK1/2 signalling in prostate cancer cells.; Authors - Jennifer Munkley
mouse	WB,IF	Front Mol Neurosci; Downregulation of Protein Tyrosine Phosphatase Receptor Type R Accounts for the Progression of Hirschsprung Disease.; Authors - Jiao Tian