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PTPRU Polyklonaler Antikörper

PTPRU Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23786-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using PTPRU Polyclonal antibody (23786-1-AP)

    WB analysis using 23786-1-AP (same clone as 23786-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23786-1-AP (PTPRU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23786-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PTPRU Polyclonal antibody (23786-1-AP)

    IF Staining of U-251 using 23786-1-AP (same clone as 23786-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PTPRU antibody (23786-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23786-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

23786-1-PBS bindet in WB, IF/ICC, Indirect ELISA PTPRU und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PTPRU fusion protein Ag20667
Vollständiger Name protein tyrosine phosphatase, receptor type, U
Berechnetes Molekulargewicht 1446 aa, 162 kDa
Beobachtetes Molekulargewicht170 kDa
GenBank-ZugangsnummerBC146655
Gene symbol PTPRU
Gene ID (NCBI) 10076
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTPRU (protein tyrosine phosphatase, receptor type, U) is also named as PCP-2,PTP-J, PTP pi, PTP-RO and R-PTP-psi. Protein tyrosine phosphatase plays a crucial role in the regulation of signal transduction during diverse cell functions, including cell activation, proliferation, differentiation, survival, and metabolic homeostasis. PTPases are classified into two groups, membrane-spanning receptor-type (R-PTPases) and cytosolictype. The human PTPRU cDNA clone encodes a polypeptide of 1,439 amino acids and the predicted molecular mass of the PTPRU protein is 162 kDa. It has reported that SCF induced tyrosine phosphorylation of the full-length PTPRU (190 kDa) and the proteolytic forms of PTPRU (100 and 80 kD). A non-tyrosine phosphorylated 73 kDa band was detected in addition to those of 190, 90, and 80 kDa. (PMID: 10397721).