PTRF Rekombinanter Antikörper

PTRF Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E15

Kat-Nr. : 82697-1-PBS

Synonyme

FKSG13, Cavin-1, Cavin 1, 3E15

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  WB analysis using 82697-1-RR (same clone as 82697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat lung using 82697-1-RR (same clone as 82697-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 82697-1-RR (same clone as 82697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82697-1-RR (same clone as 82697-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82697-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

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Geprüfte Anwendungen

Produktinformation

82697-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PTRF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PTRF fusion protein Ag13486
• Vollständiger Name: polymerase I and transcript release factor
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC008849
• Gene symbol: PTRF
• Gene ID (NCBI): 284119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.