PTRH2 Polyklonaler Antikörper

PTRH2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51006-2-AP

Synonyme

Bcl-2 inhibitor of transcription 1, BIT1, CGI 147, CGI-147, EC:3.1.1.29

---

• 

  WB analysis using 51006-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

• 

  WB analysis of Jurkat using 51006-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HepG2 using 51006-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HEK-293 using 51006-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51006-2-AP (PTRH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 51006-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 51006-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 51006-2-AP (PTRH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 51006-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 51006-2-AP (PTRH2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 51006-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PTRH2 antibody (51006-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

51006-2-AP bindet in WB, IHC, IF/ICC, ELISA PTRH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PTRH2 fusion protein Ag0839
• Vollständiger Name: peptidyl-tRNA hydrolase 2
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC006807
• Gene symbol: PTRH2
• Gene ID (NCBI): 51651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PTRH2 (Peptidyl-tRNA Hydrolase 2, also known as Bcl-2 Inhibitor of Transcription 1 or BIT1) is a highly conserved protein that plays crucial roles in various cellular processes, including protein synthesis, cell survival, and cell death.