Securin Polyklonaler Antikörper

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• KD/KO Validated

Securin Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 18040-1-AP

Synonyme

EAP1, Esp1 associated protein, HPTTG, pituitary tumor transforming 1, PTTG, PTTG1, Securin, Securin / PTTG1, Tumor transforming protein 1, TUTR1

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Galerie der Validierungsdaten

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  WB analysis using 18040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 18040-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 18040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 18040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, HepG2-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

18040-1-AP bindet in WB, IP, IHC, IF, ELISA Securin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Securin fusion protein Ag12670
• Vollständiger Name: pituitary tumor-transforming 1
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC101834
• Gene symbol: PTTG1
• Gene ID (NCBI): 9232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

Publikationen

Species	Application	Title
mouse	IHC,IF	Cell Mol Life Sci; Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.; Authors - Kyoung-In Cho
human	WB	Sci Rep; APC/C(CDC20) and APC/C play pivotal roles in the process of embryonic development in Artemia sinica.; Authors - Mengchen Zhang
mouse	WB,IF	Cell Cycle; Chronic restraint stress disturbs meiotic resumption through APC/C-mediated cyclin B1 excessive degradation in mouse oocytes.; Authors - Junyan Sun
mouse	IF	Cryobiology; Melatonin promotes in vitro maturation of vitrified-warmed mouse GV oocytes potentially by modulating MAD2 protein expression of SAC component through MTRs.; Authors - Jinyu Yang
human	WB	Open Med (Wars); The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma.; Authors - Zhenhua Zhang ; KD Validated
human	WB,IHC,IF,IP	Oncogene; Phosphorylated PTTG1 switches its subcellular distribution and promotes β-catenin stabilization and subsequent transcription activity; Authors - Xuewen Zhang ; KD Validated