Securin Rekombinanter Antikörper

Securin Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241090G10

Kat-Nr. : 83981-4-PBS

Synonyme

PTTG1, 241090G10, EAP1, Esp1-associated protein, hPTTG

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  IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83981-4-RR (same clone as 83981-4-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83981-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83981-4-RR against Human Securin were performed. The affinity constant is 0.155 nM.

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Geprüfte Anwendungen

Produktinformation

83981-4-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Securin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Securin fusion protein Ag12670
• Vollständiger Name: pituitary tumor-transforming 1
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• GenBank-Zugangsnummer: BC101834
• Gene symbol: Securin
• Gene ID (NCBI): 9232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.