Pentraxin 3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Pentraxin 3 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 13797-1-AP

Synonyme

Pentaxin related protein PTX3, Pentraxin 3, Pentraxin related protein PTX3, PTX3, TNF alpha induced protein 5, TNFAIP5, TSG 14, TSG14

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13797-1-AP

  WB result of Pentraxin 3 antibody (13797-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells.

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  WB analysis using 13797-1-AP

  HUVEC cells lysates were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13797-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13797-1-AP

  WB result of Pentraxin 3 antibody (13797-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pentraxin 3 transfected HeLa cells.

• 

  WB analysis of Jurkat using 13797-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13797-1-AP

  Immunofluorescent analysis of HepG2 cells using 13797-1-AP (Pentraxin 3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 9 publications below
• IF: See 8 publications below

Produktinformation

13797-1-AP bindet in WB, IHC, IF, ELISA Pentraxin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pentraxin 3 fusion protein Ag4624
• Vollständiger Name: pentraxin-related gene, rapidly induced by IL-1 beta
• Berechnetes Molekulargewicht: 381 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC039733
• Gene symbol: PTX3
• Gene ID (NCBI): 5806
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif99, the pentraxin domain (PMID: 15771574). Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties (PMID: 15771574). Pentraxin 3 is an acute phase glycoprotein that plays a role in the regulation of innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility and cancer (PMID: 29412047). Pentraxin 3 is a biomarker for cardiovascular disease (PMID: 22347626). Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders (PMID: 22347626; 31057548). In WB, there're two major bands, an upper signal at 80-86 kDa and a lower signal at 42 kDa, representing Pentraxin 3 dimers and monomers, respectively (PMID: 18223257).

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Urchin-like ceria nanoparticles for enhanced gene therapy of osteoarthritis; Authors - Haoyi Chen
human	WB	Nat Commun; SH3RF3 promotes breast cancer stem-like properties via JNK activation and PTX3 upregulation.; Authors - Peiyuan Zhang
human	WB	Front Cell Dev Biol; Pentraxin 3 Promotes Glioblastoma Progression by Negative Regulating Cells Autophagy.; Authors - Zeyu Wang
human,mouse	WB	Ecotoxicol Environ Saf; PTX3 alleviates hard metal-induced acute lung injury through potentiating efferocytosis.; Authors - Weiwen Yan
human	WB	J Proteomics; Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.; Authors - Sun-Xia Chen
human	WB,IHC	CNS Neurosci Ther; PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.; Authors - Hao Zhang ; KD Validated

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