PUF-A,KIAA0020 Monoklonaler Antikörper

PUF-A,KIAA0020 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G4B7

Kat-Nr. : 67941-1-Ig

Synonyme

HLA HA8, KIAA0020, PEN, PUF6, PUF-A, PUM3, XTP5

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Galerie der Validierungsdaten

• 

  WB analysis using 67941-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HSC-T6 using 67941-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67941-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67941-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67941-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67941-1-Ig (KIAA0020 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67941-1-Ig bindet in WB, ELISA PUF-A,KIAA0020 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PUF-A,KIAA0020 fusion protein Ag31324
• Vollständiger Name: KIAA0020
• Berechnetes Molekulargewicht: 648 aa, 74 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC016137
• Gene symbol: KIAA0020
• Gene ID (NCBI): 9933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.