PUM1 Polyklonaler Antikörper

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• KD/KO Validated

PUM1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 26256-1-AP

Synonyme

HSPUM, KIAA0099, PUM1, PUMH, PUMH1, Pumilio 1, Pumilio homolog 1, pumilio homolog 1 (Drosophila), PUML1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 26256-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26256-1-AP (PUM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 26256-1-AP

  IP Result of anti-PUM1 (IP:26256-1-AP, 4ug; Detection:26256-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IF Staining of MCF-7 using 26256-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 26256-1-AP (PUM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

26256-1-AP bindet in WB, IP, IHC, IF, ELISA PUM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PUM1 fusion protein Ag24651
• Vollständiger Name: pumilio homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1186 aa, 126 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC013398
• Gene symbol: PUM1
• Gene ID (NCBI): 9698
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Biochem Biophys Res Commun; PUM1 promotes ovarian cancer proliferation, migration and invasion.; Authors - Xue Guan ; KD Validated
human	WB	Carcinogenesis; PAUF/ZG16B promotes colorectal cancer progression through alterations of the mitotic functions and the Wnt/β-catenin pathway.; Authors - Beatriz Escudero-Paniagua