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PUM1 Rekombinanter Antikörper

PUM1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250792G4

Kat-Nr. : 86129-1-PBS

Synonyme

HsPUM, KIAA0099, PUMH, PUMH1, Pumilio 1

Filter:
  • Western Blot (WB) analysis of rat brain tissue using PUM1 Recombinant monoclonal antibody (86129-1-RR)

    WB analysis of rat brain using 86129-1-RR (same clone as 86129-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PUM1 Recombinant monoclonal antibody (86129-1-RR)

    WB analysis using 86129-1-RR (same clone as 86129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PUM1 Recombinant monoclonal antibody (86129-1-RR)

    IF Staining of HeLa using 86129-1-RR (same clone as 86129-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PUM1 antibody (86129-1-RR, Clone: 250792G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86129-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86129-1-RR

    Affinity and Kinetic Characterization of 86129-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86129-1-RR against Human PUM1 were performed. The affinity constant is 2.46 nM.

Geprüfte Anwendungen

Produktinformation

86129-1-PBS bindet in WB, IF/ICC, Indirect ELISA PUM1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PUM1 fusion protein Ag24651
Vollständiger Name pumilio homolog 1 (Drosophila)
Berechnetes Molekulargewicht 1186 aa, 126 kDa
Beobachtetes Molekulargewicht140 kDa
GenBank-ZugangsnummerBC013398
Gene symbol PUM1
Gene ID (NCBI) 9698
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.