PUM2 Polyklonaler Antikörper

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• KD/KO Validated

PUM2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11586-1-AP

Synonyme

KIAA0235, PUM2, PUMH2, Pumilio 2, Pumilio homolog 2, pumilio homolog 2 (Drosophila), PUML2

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  WB analysis of mouse testis using 11586-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11586-1-AP (PUM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

11586-1-AP bindet in WB, ELISA PUM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PUM2 fusion protein Ag2179
• Vollständiger Name: pumilio homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 95 kDa, 140 kDa
• GenBank-Zugangsnummer: BC024218
• Gene symbol: PUM2
• Gene ID (NCBI): 23369
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PUM2 (Human Pumilio homology protein 2), a member of the PUF protein family, binds to 3′ untranslated regions (3′ UTRs) of mRNA and to repress gene expression by controlling cytoplasmic polyadenylation and affecting mRNA translation [PMID:12459267]. When translationally dormant, PUM2, CPEB (CPE-binding protein) and Maskin form a complex on maternal cyclin B1 mRNA, precluding the formation of the active translation initiation complex [PMID:15687499]. PUM2 has been found to play a role in the mammalian nervous system, in particular in regulating morphology, arborization and excitability of neuronal dendrites [PMID:22016787]. The calculated molecular weight of PUM2 is 114 kDa, but the modified PUM2 protein is about 140 kDa.

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.; Authors - Lansheng Zhang ; KD Validated