PUMA Polyklonaler Antikörper

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• KD/KO Validated

PUMA Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(73)

Rezensionen (1)

Kat-Nr. : 55120-1-AP

Synonyme

BBC3, BBC 3, Bcl-2-binding component 3, isoforms 1/2, JFY 1, JFY1

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  WB analysis using 55120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55120-1-AP (PUMA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 55120-1-AP

  IP result of anti-PUMA (IP:55120-1-AP, 4ug; Detection:55120-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human testis using 55120-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 55120-1-AP (PUMA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 55120-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 55120-1-AP (PUMA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 55120-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55120-1-AP (PUMA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 70 publications below
• IHC: See 4 publications below

Produktinformation

55120-1-AP bindet in WB, IHC, IP, ELISA PUMA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: BCL2 binding component 3
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: NM_014417
• Gene symbol: PUMA
• Gene ID (NCBI): 27113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PUMA, also named as JFY-1 and BBC3, belongs to the Bcl-2 family. It is a critical mediator of p53-dependent and -independent apoptosis induced by a wide variety of stimuli. It serves as a proximal signaling molecule whose expression is regulated by transcription factors in response to these stimuli. PUMA transduces death signals primarily to the mitochondria, where it acts indirectly on the Bcl-2 family members Bax and/or Bak by relieving the inhibition imposed by antiapoptotic members. It directly binds and antagonizes all known antiapoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. PUMA ablation or inhibition leads to apoptosis deficiency underlying increased risks for cancer development and therapeutic resistance. It is a general sensor of cell death stimuli and a promising drug target for cancer therapy and tissue damage. It is essential mediator of p53-dependent and p53-independent apoptosis (PMID: 19641508). Catalog #55120-1-AP can recognize PUMA alpha 21-24 kDa and PUMA beta 15-18 kDa.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao
human	WB	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
mouse	WB	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
mouse	WB	Cell Death Differ; Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.; Authors - Kunlun Yin
human	WB	Environ Pollut; Insight into the negative impact of ionic liquid: A cytotoxicity mechanism of 1-methyl-3-octylimidazolium bromide.; Authors - Junguo Ma

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (03-01-2023)

Very good working, pvdf membrane, blocking solution skim milk. One sharp band at the expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

PUMA Antibody Western Blot validation (1:2500 dilution) in Human pulmonary artery smooth muscle cells (Cat no:55120-1-AP)