PURA Monoklonaler Antikörper

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• KD/KO Validated

PURA Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1C6G4

Kat-Nr. : 67305-1-Ig

Synonyme

PUR ALPHA, PUR1, PURA, PURALPHA

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  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67305-1-Ig

  WB result of PURA antibody (67305-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PURA transfected HepG2 cells.

• 

  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Plazenta-Gewebe, L02-Zellen, LNCaP-Zellen, Maushirngewebe, Maus-Cerebellum-Gewebe, Hausschwein-Hirngewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

67305-1-Ig bindet in WB, IF, ChIP, RIP, ELISA PURA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PURA fusion protein Ag12164
• Vollständiger Name: purine-rich element binding protein A
• Berechnetes Molekulargewicht: 322 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC036087
• Gene symbol: PURA
• Gene ID (NCBI): 5813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.

Publikationen

Species	Application	Title
human	RIP,IF,ChIP	Int J Biol Macromol; MTA1-DT promotes endometrial cancer growth by modulating G2/M-related gene transcription via PURα; Authors - Yiting Liu