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PURG Rekombinanter Antikörper

PURG Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241504E7

Kat-Nr. : 84399-3-RR

Synonyme

241504E7, PURG A, PURG B

Filter:
  • Western Blot (WB) analysis of various lysates using PURG Recombinant antibody (84399-3-RR)

    WB analysis using 84399-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84399-3-RR (PURG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PURG Recombinant antibody (84399-3-RR)

    IF Staining of HeLa using 84399-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PURG antibody (84399-3-RR, Clone: 241504E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84399-3-RR

    Affinity and Kinetic Characterization of 84399-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84399-3-RR against Human PURG were performed. The affinity constant is 0.328 nM.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

84399-3-RR bindet in WB, IF/ICC, ELISA PURG und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PURG fusion protein Ag15820
Vollständiger Name purine-rich element binding protein G
Berechnetes Molekulargewicht 347 aa, 40 kDa
Beobachtetes Molekulargewicht40 kDa
GenBank-ZugangsnummerBC106708
Gene symbol PURG
Gene ID (NCBI) 29942
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PURG (Purine-rich element-binding protein G) is a member of the PUR protein family. It has 2 isoforms with a molecular weight of 37-40 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PURG antibody 84399-3-RRProtokoll herunterladen
IF protocol for PURG antibody 84399-3-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen