PUS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PUS1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11512-1-PBS

Synonyme

EC:5.4.99.-, EC:5.4.99.12, MLASA, PP8985, pseudouridylate synthase 1

---

• 

  WB analysis of mouse lung using 11512-1-AP (same clone as 11512-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11512-1-AP (same clone as 11512-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11512-1-AP (same clone as 11512-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 11512-1-AP (same clone as 11512-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 11512-1-AP (same clone as 11512-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PUS1 antibody (11512-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11512-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

11512-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PUS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PUS1 fusion protein Ag2071
• Vollständiger Name: pseudouridylate synthase 1
• Berechnetes Molekulargewicht: 427 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC019320
• Gene symbol: PUS1
• Gene ID (NCBI): 80324
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.