CD155/PVR Polyklonaler Antikörper

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• KD/KO Validated

CD155/PVR Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 27486-1-AP

Synonyme

PVR, Nectin like protein 5, NECL-5, NECL5, Necl 5

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  WB analysis of A549 using 27486-1-AP

  WB result of CD155/PVR antibody (27486-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CD155/PVR knockout A549 cells.

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  WB analysis of HT-1080 using 27486-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (CD155/PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27486-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 27486-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 27486-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27486-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (CD155/PVR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, A549-Zellen, HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

27486-1-AP bindet in WB, IHC, IF, ELISA CD155/PVR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD155/PVR fusion protein Ag26657
• Vollständiger Name: poliovirus receptor
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC015542
• Gene symbol: CD155/PVR
• Gene ID (NCBI): 5817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

Publikationen

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
		Acta Pharm Sin B; PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability; Authors - Jiaojiao Yu
human	IF	Sci Adv; Ara-C suppresses H3 K27-altered spinal cord diffuse midline glioma growth and enhances immune checkpoint blockade sensitivity; Authors - Bo Pang