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  • KD/KO Validated

Nectin-1/PVRL1 Polyklonaler Antikörper

Nectin-1/PVRL1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 24713-1-AP

Synonyme

CD111, CLPED1, ED4, Herpes virus entry mediator C, Herpesvirus entry mediator C, Herpesvirus Ig like receptor, HIgR, HVEC, nectin 1, Nectin-1, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK 12



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe, SH-SY5Y-Zellen, SKOV-3-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

24713-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Nectin-1/PVRL1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Nectin-1/PVRL1 fusion protein Ag20074
Vollständiger Name poliovirus receptor-related 1 (herpesvirus entry mediator C)
Berechnetes Molekulargewicht 517 aa, 57 kDa
Beobachtetes Molekulargewicht90 kDa, 57 kDa
GenBank-ZugangsnummerBC104948
Gene symbol Nectin-1
Gene ID (NCBI) 5818
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectins are a family of immunoglobulin-like cell adhesion molecules comprising four members, nectin-1 to nectin-4, each consisting of an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain (PMID: 12901789, 18703497). Nectins are involved in cell adhesion, migration, polarization, morphogenesis, proliferation, differentiation, and survival. Nectin-1, also known as PVRL1, HVEC and CD111, is widely expressed in a variety of cell types including epithelial cells, neurons, and fibroblasts (PMID: 12901789). It promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Nectin-1 acts as a receptor for several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1) (PMID: 9616127). Nectin-1 is a glycoprotein, the glycosylated form has a larger apparent molecular weight than the calculated molecular weight of 57 kDa (PMID: 12376527; 15731277).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Nectin-1/PVRL1 antibody 24713-1-APProtokoll herunterladen
IHC protocol for Nectin-1/PVRL1 antibody 24713-1-APProtokoll herunterladenl
IF protocol for Nectin-1/PVRL1 antibody 24713-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

PLoS Pathog

Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 1.

Authors - Hiroki Takeuchi
humanIHC,WB

Front Oncol

High expression of nectin-1 indicates a poor prognosis and promotes metastasis in hepatocellular carcinoma

Authors - Xuequan Wang
  • KD Validated
mouseIHC

Mol Ther Oncolytics

Oncolytic activity of naturally attenuated herpes-simplex virus HF10 against an immunocompetent model of oral carcinoma.

Authors - Gaku Takano
humanIHC

Heliyon

Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study

Authors - Lingyan Chen

bioRxiv

Herpes Simplex Virus 1 Envelope Glycoprotein C Shields Glycoprotein D to Protect Virions from Entry-Blocking Antibodies

Authors - McKenna A Hull

J Virol

Neuropilin-1 Facilitates Pseudorabies Virus Replication and Viral Glycoprotein B Promotes Its Degradation in a Furin-Dependent Manner

Authors - Meng Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Morgan (Verified Customer) (08-09-2021)

This PVRL1 worked well with the ovarian cancer cell line OV8. It was more difficult to get a clear detection in the ovarian cancer cell line OV90, but we believe a similar trend was apparent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: OV8 and 0V90
Nectin-1/PVRL1 Antibody Western Blot validation (1:1000 dilution) in OV8 and 0V90 (Cat no:24713-1-AP)