Nectin 3 Rekombinanter Antikörper

Nectin 3 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242804D5

Kat-Nr. : 85833-4-PBS

Synonyme

Nectin-3, PVRL3, CD113, Nectin cell adhesion molecule 3, poliovirus receptor related 3

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  WB analysis using 85833-4-RR (same clone as 85833-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85833-4-RR (PVRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85833-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02144-3

  Sandwich ELISA standard curve of MP02144-3, Human Nectin 3 Recombinant Matched Antibody Pair - PBS only. 85833-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2657. 85833-3-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  Sample test of MP02144-3

  The mean Nectin 3 concentration was determined to be 269.06 ng/mL in MDA-MB-231 cell extract based on a 1.6 mg/mL extract load and 377.13 ng/mL in HCT116 cell extract based on a 1.3 mg/mL extract load.

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Geprüfte Anwendungen

Produktinformation

85833-4-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test Nectin 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nectin 3 fusion protein Eg2657
• Vollständiger Name: poliovirus receptor-related 3
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_015480.3
• Gene symbol: Nectin-3
• Gene ID (NCBI): 25945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin 3, also known as CD113, is a member of the nectin family of cell adhesion molecules. It plays a crucial role in cell-cell adhesion and the formation of adherens junctions, which are essential for maintaining tissue integrity and cell polarity. Nectin 3 can form homophilic and heterophilic interactions, meaning it can bind to itself or other nectin family members, such as Nectin-2 (PMID: 10744716; 22902367). These interactions are important for processes like cell migration, tissue development, and immune cell transmigration (PMID: 12901789; 24116228).