ASC/TMS1 Polyklonaler Antikörper

ASC/TMS1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 30641-1-AP

Synonyme

PYCARD, PYD and CARD domain containing, masc, CARD5, Asc

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  WB analysis using 30641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 30641-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PYCARD antibody (30641-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Maus-Thymusgewebe, Rattenmilzgewebe, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

30641-1-AP bindet in WB, IF/ICC, ELISA ASC/TMS1 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASC/TMS1 fusion protein Ag32519
• Vollständiger Name: PYD and CARD domain containing
• Berechnetes Molekulargewicht: 21KD
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: NM_023258
• Gene symbol: Pycard
• Gene ID (NCBI): 66824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

Publikationen

Species	Application	Title
mouse	WB	Food Sci Biotechnol; Geraniol alleviates liver injury induced by bisphenol A via modulating NLRP3/caspase-1 pathway and gut microbiota in mice model; Authors - Bangyuan Zhou
human,mouse	WB	Int Immunopharmacol; Deletion of BRCC3 ameliorates airway inflammation in asthma by inhibiting the activation of NLRP3 inflammasome; Authors - Hao Wang
mouse	WB	Tissue Cell; Baicalin reduced vandetanib induced myocardial injury by regulating redox balance and NLRP3 inflammasome pathway; Authors - Fen Wang
rat	WB	Front Pharmacol; Edaravone dexborneol protected neurological function by targeting NRF2/ARE and NF-κB/AIM2 pathways in cerebral ischemia/reperfusion injury; Authors - Hui Zhang