ASC/TMS1 Rekombinanter Antikörper

ASC/TMS1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240908D5

Kat-Nr. : 83858-3-RR

Synonyme

Pycard, PYD and CARD domain containing, masc, CARD5, Asc

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  WB analysis using 83858-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83858-3-RR (PYCARD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83858-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83858-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83858-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83858-3-RR against Mouse PYCARD were performed. The affinity constant is 3.58 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, Mauslungengewebe, Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83858-3-RR bindet in WB, IHC, ELISA ASC/TMS1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ASC/TMS1 fusion protein Ag32519
• Vollständiger Name: PYD and CARD domain containing
• Berechnetes Molekulargewicht: 21KD
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: NM_023258
• Gene symbol: Pycard
• Gene ID (NCBI): 66824
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).