PYCR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PYCR1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

44

Rezensionen (1)

Kat-Nr. : 13108-1-AP

Synonyme

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

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Galerie der Validierungsdaten

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  WB analysis using 13108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 13108-1-AP

  WB result of PYCR1 antibody (13108-1-AP, 1:5000) with si-Control and si-PYCR1 transfected HeLa cells.

• 

  WB analysis of mouse embryo using 13108-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13108-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13108-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13108-1-AP

  IP Result of anti-PYCR1 (IP:13108-1-AP, 3ug; Detection:13108-1-AP 1:700) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 13108-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13108-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13108-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13108-1-AP (PYCR1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 13108-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 13108-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PYCR1 antibody 13108-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Hirngewebe, Mausembryogewebe, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 39 publications below
• IHC: See 10 publications below
• IF: See 10 publications below
• IP: See 5 publications below

Produktinformation

13108-1-AP bindet in WB, IP, IHC, IF, ELISA PYCR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYCR1 fusion protein Ag3764
• Vollständiger Name: pyrroline-5-carboxylate reductase 1
• Berechnetes Molekulargewicht: 319 aa, 33.8 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 35 kDa
• GenBank-Zugangsnummer: BC022244
• Gene symbol: PYCR1
• Gene ID (NCBI): 5831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Publikationen

Species	Application	Title
human	WB,IHC	Nature; Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.; Authors - Fabricio Loayza-Puch ; KO Validated
human	WB	Nature; Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.; Authors - Possemato Richard R
zebrafish	WB,IF	Nat Genet; Mutations in PYCR1 cause cutis laxa with progeroid features.; Authors - Reversade Bruno B
mouse	WB	Cell Metab; Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.; Authors - Sukru Anil Dogan
human	IHC	J Hepatol; Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.; Authors - Zhaobing Ding
human,mouse	WB,IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (02-05-2020)

The antibody stained the glomerulus of the mouse kidney.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

PYCR1 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:13108-1-AP)