PYCR1 Polyklonaler Antikörper

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• KD/KO Validated

PYCR1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22150-1-PBS

Synonyme

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  WB analysis of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 22150-1-AP (same clone as 22150-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

  IP result of anti-PYCR1 (IP:22150-1-AP, 4ug; Detection:22150-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22150-1-AP (same clone as 22150-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22150-1-AP (PYCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22150-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22150-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA PYCR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYCR1 fusion protein Ag17745
• Vollständiger Name: pyrroline-5-carboxylate reductase 1
• Berechnetes Molekulargewicht: 319 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC022244
• Gene symbol: PYCR1
• Gene ID (NCBI): 5831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody may have cross reaction to PYCR2 due to the high homology.