PYCR1 Monoklonaler Antikörper

PYCR1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1H2G1

Kat-Nr. : 66510-1-Ig

Synonyme

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

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  WB analysis using 66510-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour.

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  IHC staining of human breast cancer using 66510-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66510-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66510-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HEK-293-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

66510-1-Ig bindet in WB, IHC, IF/ICC, ELISA PYCR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PYCR1 fusion protein Ag17919
• Vollständiger Name: pyrroline-5-carboxylate reductase 1
• Berechnetes Molekulargewicht: 319 aa, 33.8 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 35 kDa
• GenBank-Zugangsnummer: BC022244
• Gene symbol: PYCR1
• Gene ID (NCBI): 5831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.

Publikationen

Species	Application	Title
human	WB	Cancer Med; Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma; Authors - Yimin Sun
human	IHC	Appl Immunohistochem Mol Morphol; PYCR1, BANF1, and STARD8 Expression in Gastric Carcinoma: A Clinicopathologic, Prognostic, and Immunohistochemical Study; Authors - Ola A Harb