PYCR1 Monoklonaler Antikörper

PYCR1 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H2G1

Kat-Nr. : 66510-1-PBS

Synonyme

P5CR 1, P5CR, P5C reductase 1, P5C, EC:1.5.1.2

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  WB analysis using 66510-1-Ig (same clone as 66510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66510-1-Ig (PYCR1 antibody) at dilution of 1:3000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66510-1-Ig (PYCR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66510-1-Ig (same clone as 66510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR1 antibody (66510-1-Ig, Clone: 1H2G1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66510-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50786-1

  Cytometric bead array standard curve of MP50786-1, PYCR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66510-2-PBS. Detection antibody: 66510-1-PBS. Standard:Ag17919. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66510-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA PYCR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PYCR1 fusion protein Ag17919
• Vollständiger Name: pyrroline-5-carboxylate reductase 1
• Berechnetes Molekulargewicht: 319 aa, 33.8 kDa
• Beobachtetes Molekulargewicht: 33 kDa, 35 kDa
• GenBank-Zugangsnummer: BC022244
• Gene symbol: PYCR1
• Gene ID (NCBI): 5831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.