PYCR2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PYCR2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (4)

Kat-Nr. : 17146-1-AP

Synonyme

P5C reductase 2, P5CR 2, P5CR2, PYCR2

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Galerie der Validierungsdaten

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  WB analysis using 17146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17146-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 17146-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17146-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17146-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17146-1-AP (PYCR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17146-1-AP (PYCR2 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17146-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17146-1-AP (PYCR2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 17146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PYCR2 antibody (17146-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1500-1:6000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

17146-1-AP bindet in WB, IHC, IF, ELISA PYCR2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Südliche Grünmeerkatze
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYCR2 fusion protein Ag10918
• Vollständiger Name: pyrroline-5-carboxylate reductase family, member 2
• Berechnetes Molekulargewicht: 320 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC020553
• Gene symbol: PYCR2
• Gene ID (NCBI): 29920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.

Publikationen

Species	Application	Title
human	IF	Cell; Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.; Authors - Qiang Gao
human	WB	Sci Adv; Loss of attachment promotes proline accumulation and excretion in cancer cells; Authors - Steven E Pilley
mouse	WB	EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human	WB	EMBO J; Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.; Authors - Zhen Chen
human	WB	Cell Rep; Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.; Authors - Kate E R Hollinshead ; KD Validated
African green monkey	WB	Virol Sin; African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.; Authors - Sheng Chen ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

balawant (Verified Customer) (12-25-2023)

I have used this antibody . This antibody is working great.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for western blot and 1:700 for IHC
• Cell Tissue Type: colon tissue of mice and human

FH

Balawant (Verified Customer) (08-06-2022)

This antibody is working great. I have used this antibody for tissue lysate and cell lysate. it has no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: CRC cell line

FH

Iram (Verified Customer) (07-03-2020)

Giving a very sharp and clean result

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human