PYCR2 Monoklonaler Antikörper

PYCR2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G7D9

Kat-Nr. : 68488-1-Ig

Synonyme

FLJ54750, P5C reductase 2, P5CR 2, P5CR2, PYCR2

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  WB analysis using 68488-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 68488-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68488-1-Ig (PYCR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, K-562-Zellen, LNCaP-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68488-1-Ig bindet in WB, ELISA PYCR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PYCR2 fusion protein Ag10923
• Vollständiger Name: pyrroline-5-carboxylate reductase family, member 2
• Berechnetes Molekulargewicht: 320 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC020553
• Gene symbol: PYCR2
• Gene ID (NCBI): 29920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.