PYCRL Polyklonaler Antikörper

PYCRL Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 16008-1-AP

Synonyme

PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3

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  WB analysis using 16008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, MCF-7-Zellen, Mausnierengewebe, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

16008-1-AP bindet in WB, ELISA PYCRL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYCRL fusion protein Ag8716
• Vollständiger Name: pyrroline-5-carboxylate reductase-like
• Berechnetes Molekulargewicht: 274 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC007993
• Gene symbol: PYCRL
• Gene ID (NCBI): 65263
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433)

Publikationen

Species	Application	Title
human	WB	Sci Rep; Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.; Authors - Wei Liu ; KD Validated
human	WB	Vet Microbiol; Host factor cyclophilin B affects Orf virus replication by interacting with viral ORF058 protein.; Authors - Yanlong Zhou