PYGB Polyklonaler Antikörper

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• KD/KO Validated

PYGB Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 12075-1-AP

Synonyme

phosphorylase, glycogen; brain, GPBB, EC:2.4.1.1

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  WB analysis of human brain using 12075-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12075-1-AP

  WB result of PYGB antibody (12075-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

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  WB analysis of rat brain using 12075-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12075-1-AP

  IP result of anti-PYGB (IP:12075-1-AP, 4ug; Detection:12075-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat brain using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Ovarialkarzinomgewebe, Mausherzgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IP: See 2 publications below

Produktinformation

12075-1-AP bindet in WB, IHC, IP, ELISA PYGB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYGB fusion protein Ag2732
• Vollständiger Name: phosphorylase, glycogen; brain
• Berechnetes Molekulargewicht: 843 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC017045
• Gene symbol: PYGB
• Gene ID (NCBI): 5834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The three main subtypes of GP found in human tissues-brain-type glycogen phosphorylase (PYGB), liver-type glycogen phosphorylase (PYGL), and muscle-type glycogen phosphorylase (PYGM)-differ in function, structure, and tissue distribution. PYGB (Brain‑type glycogen phosphorylase) is a glycogen phosphorylase and is primarily localized in adult brain tissues and embryo liver tissues, whose function is to provide energy to organisms (PMID: 30106110). PYGB has been observed to be overexpressed in many types of cancer and tumor cell lines and is expected to be a novel target for the diagnosis and treatment of various cancers (PMID: 38334681).

Publikationen

Species	Application	Title
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
human	WB	Mol Cell Proteomics; Multi-omics integration reveals the landscape of pro-metastasis metabolism in hepatocellular carcinoma.; Authors - Yongmei Li
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu
human	WB	Front Cell Dev Biol; Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.; Authors - Guangying Cui
mouse	WB	J Cell Mol Med; PMID: 21251210; Authors - Xi Lei L

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

works fine

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer