PYGB Polyklonaler Antikörper

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• KD/KO Validated

PYGB Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 55380-1-AP

Synonyme

phosphorylase, glycogen; brain, PYGB

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Galerie der Validierungsdaten

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  WB analysis of A549 using 55380-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55380-1-AP

  WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

• 

  WB analysis of A549 using 55380-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55380-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55380-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55380-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 55380-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55380-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 55380-1-AP

  Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, MCF-7-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

55380-1-AP bindet in WB, IHC, IF, ELISA PYGB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phosphorylase, glycogen; brain
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 97-105 kDa
• GenBank-Zugangsnummer: NM_002862
• Gene symbol: PYGB
• Gene ID (NCBI): 5834
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.

Publikationen

Species	Application	Title
mouse	WB	Front Mol Neurosci; Brain-Type Glycogen Phosphorylase Is Crucial for Astrocytic Glycogen Accumulation in Chronic Social Defeat Stress-Induced Depression in Mice.; Authors - Yuanyuan Zhu
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan