PYGL Polyklonaler Antikörper

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• KD/KO Validated

PYGL Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(39)

Rezensionen (1)

Kat-Nr. : 15851-1-AP

Synonyme

Glycogen phosphorylase, liver form, EC:2.4.1.1

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• 

  WB analysis using 15851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15851-1-AP (PYGL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15851-1-AP

  IP result of anti-PYGL (IP:15851-1-AP, 4ug; Detection:15851-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human kidney using 15851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15851-1-AP (PYGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15851-1-AP (PYGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15851-1-AP (PYGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15851-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PYGL antibody (15851-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausherzgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 34 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

15851-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PYGL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Eichh?rnchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PYGL fusion protein Ag8664
• Vollständiger Name: phosphorylase, glycogen, liver
• Berechnetes Molekulargewicht: 846 aa, 97 kDa
• Beobachtetes Molekulargewicht: 97 kDa
• GenBank-Zugangsnummer: BC009895
• Gene symbol: PYGL
• Gene ID (NCBI): 5836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Nature; Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes.; Authors - Sophia Maschalidi
		Nat Med; PMID: 30150719; Authors - Roger W Hunter
human	WB	Cell Metab; Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.; Authors - Marion Curtis
human,mouse	WB	Cell Metab; Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.; Authors - Phyu M Thwe
mouse	WB	J Clin Invest; Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.; Authors - Miguel A Lanaspa
human	IHC,IF,WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer