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PYGL Monoklonaler Antikörper

PYGL Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G12D8

Kat-Nr. : 66769-1-PBS

Synonyme

Glycogen phosphorylase, liver form, EC:2.4.1.1, 1G12D8

Filter:
  • Western Blot (WB) analysis of HSC-T6 cells using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis of HSC-T6 using 66769-1-Ig (same clone as 66769-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis using 66769-1-Ig (same clone as 66769-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66769-1-Ig (PYGL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PYGL Monoclonal antibody (66769-1-Ig)

    WB analysis of HeLa using 66769-1-Ig (same clone as 66769-1-PBS)

    WB result of PYGL antibody (66769-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGL transfected HeLa cells. This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PYGL Monoclonal antibody (66769-1-Ig)

    IF Staining of U-251 using 66769-1-Ig (same clone as 66769-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PYGL antibody (66769-1-Ig, Clone: 1G12D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66769-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66769-1-PBS bindet in WB, IF/ICC, Indirect ELISA PYGL und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PYGL fusion protein Ag8550
Vollständiger Name phosphorylase, glycogen, liver
Berechnetes Molekulargewicht 846 aa, 97 kDa
Beobachtetes Molekulargewicht 97 kDa
GenBank-ZugangsnummerBC009895
Gene symbol PYGL
Gene ID (NCBI) 5836
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycogen phosphorylase L (PYGL) is one of the gene related to hypoxia metabolism and was found to be up-regulated in head and neck squamous cell carcinomas (HNSCCs) and breast cancers. Glycogen synthases (GSs) and glycogen phosphorylases (GPs) catalyze the key steps of glycogen synthesis and breakdown, while glycogen phosphorylase has three isoforms: PYGM (muscle), PYGL (liver), and PYGB (brain) (PMID: 37063425, 9529348). High PYGL expression plays an independent role in predicting the poor prognosis of glioma patients (PMID: 34177761).