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PYROXD1 Rekombinanter Antikörper

PYROXD1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241639D7

Kat-Nr. : 84289-7-PBS

Synonyme

241639D7, pyridine nucleotide disulphide oxidoreductase domain 1

Filter:
  • Western Blot (WB) analysis of HeLa cells using PYROXD1 Recombinant antibody (84289-7-RR)

    WB analysis of HeLa using 84289-7-RR (same clone as 84289-7-PBS)

    WB result of PYROXD1 antibody (84289-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells. This data was developed using the same antibody clone with 84289-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PYROXD1 Recombinant antibody (84289-7-RR)

    WB analysis using 84289-7-RR (same clone as 84289-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84289-7-RR (PYROXD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84289-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84289-7-RR

    Affinity and Kinetic Characterization of 84289-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84289-7-RR against Human PYROXD1 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84289-7-PBS bindet in WB, Indirect ELISA PYROXD1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PYROXD1 fusion protein Ag34768
Vollständiger Name pyridine nucleotide-disulphide oxidoreductase domain 1
Beobachtetes Molekulargewicht55~60 kDa
GenBank-ZugangsnummerBC021662
Gene symbol PYROXD1
Gene ID (NCBI) 79912
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.