PYROXD1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PYROXD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241639D7

Kat-Nr. : 84289-7-RR

Synonyme

241639D7, pyridine nucleotide disulphide oxidoreductase domain 1

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  WB analysis of HeLa using 84289-7-RR

  WB result of PYROXD1 antibody (84289-7-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYROXD1 transfected HeLa cells.

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  WB analysis using 84289-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84289-7-RR (PYROXD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84289-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84289-7-RR against Human PYROXD1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen, COLO 320-Zellen, PC-3-Zellen, THP-1-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84289-7-RR bindet in WB, ELISA PYROXD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PYROXD1 fusion protein Ag34768
• Vollständiger Name: pyridine nucleotide-disulphide oxidoreductase domain 1
• Beobachtetes Molekulargewicht: 55~60 kDa
• GenBank-Zugangsnummer: BC021662
• Gene symbol: PYROXD1
• Gene ID (NCBI): 79912
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

pyridine nucleotide-disulfide oxidoreductase domain 1 (PYROXD1) is a putative oxidoreductase that co-evolved with the tRNA-LC and is essential for its activity in cells and in vivo. PYROXD1 oxidizes NAD(P)H to NAD(P)+ with tightly controlled kinetics, reducing one molecule of O2 into H2O2 per turnover. PYROXD1 is expressed across a range of tissue types in humans, including skeletal muscle, and is conserved across many species.