Pregnancy Zone Protein Polyklonaler Antikörper

Pregnancy Zone Protein Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21742-1-AP

Synonyme

PZP, pregnancy-zone protein

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  WB analysis using 21742-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 21742-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (Pregnancy Zone Protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21742-1-AP (Pregnancy Zone Protein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21742-1-AP (pregnancy zone protein antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21742-1-AP bindet in WB, IHC, IF, ELISA Pregnancy Zone Protein und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pregnancy Zone Protein fusion protein Ag16471
• Vollständiger Name: pregnancy-zone protein
• Berechnetes Molekulargewicht: 1482 aa, 164 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC111756
• Gene symbol: Pregnancy Zone Protein
• Gene ID (NCBI): 5858
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pregnancy Zone Protein (PZP) is an immunosuppressive protein that is expressed by the placenta. The PZP is a thiol ester-containing alpha macroglobulin protein that controls the proteolytic pathways during pregnancy and contributes to maintaining the immunologically privileged status of the fetus. Recently PZP over-expression has been described as a novel biomarker for predicting the prognosis of hepatocellular carcinoma and its high expression has been reported in solid tumors.

Publikationen

Species	Application	Title
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu
	WB,IHC,IF	Stem Cells Transl Med; Mesenchymal stromal cells-derived small extracellular vesicles protect against UV-induced photoaging via regulating pregnancy zone protein; Authors - Zixuan Sun
human	WB	J Gene Med; Integrated Single-Cell Transcriptome and eQTL Analyses Reveal the Role of PZP in Aging and Chronic Kidney Disease; Authors - Xinhui Huang