Pan-Keratin (TypeⅡ) Rekombinanter Antikörper

Pan-Keratin (TypeⅡ) Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

251040F7

Kat-Nr. : 86370-1-RR

Synonyme

Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins, Type II keratin

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  WB analysis using 86370-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 86370-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 86370-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 86370-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 86370-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86370-1-RR (Pan-Keratin (TypeⅡ) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 86370-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 86370-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human cervical cancer using 86370-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (TypeⅡ) antibody (86370-1-RR, Clone: 251040F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HT-29-Zellen, Maushautgewebe, Ratten-Kolongewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86370-1-RR bindet in WB, IHC, IF-P, ELISA Pan-Keratin (TypeⅡ) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Pan-Keratin (TypeⅡ)
• Beobachtetes Molekulargewicht: 52-68 kDa
• Gene ID (NCBI): Pan-Keratin (TypeⅡ)
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86.