Pan-Keratin (Type I) Rekombinanter Antikörper

Pan-Keratin (Type I) Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251039C1

Kat-Nr. : 86238-2-PBS

Synonyme

pan-CK, Pan-CK (Type I), Pan-Keratin, Type I keratin, Type I Keratin (Acidic)

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  WB analysis using 86238-2-RR (same clone as 86238-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsil using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsil using 86238-2-RR (same clone as 86238-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human cervical cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 86238-2-RR (same clone as 86238-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86238-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86238-2-PBS bindet in WB, IHC, IF-P, Indirect ELISA Pan-Keratin (Type I) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Pan-Keratin (Type I)
• Beobachtetes Molekulargewicht: 45-65 kDa
• Gene ID (NCBI): Pan-Keratin (Type I)
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.