Pan-Keratin (Type I) Rekombinanter Antikörper

Pan-Keratin (Type I) Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

251039C1

Kat-Nr. : 86238-2-RR

Synonyme

pan-CK, Pan-CK (Type I), Pan-Keratin, Type I keratin, Type I Keratin (Acidic)

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  WB analysis using 86238-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Kidney(renal cell) Cancer using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 86238-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86238-2-RR (Pan-Keratin (Type I) antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human cervical cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Pan-Keratin (TypeⅡ) antibody 86370-1-RR labeled with FlexAble 2.0 CoraLite® Plus 488 Kit (KFA501, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skin using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 86238-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Pan-Keratin (Type I) antibody (86238-2-RR, Clone: 251039C1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HaCaT-Zellen, A431-Zellen, HT-29-Zellen, Maushautgewebe, Ratten-Kolongewebe, Rattenhautgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Tonsillengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86238-2-RR bindet in WB, IHC, IF-P, ELISA Pan-Keratin (Type I) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Pan-Keratin (Type I)
• Beobachtetes Molekulargewicht: 45-65 kDa
• Gene ID (NCBI): Pan-Keratin (Type I)
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type I keratins (or Type I cytokeratins) are acidic, low molecular weight (40-64 kDa) proteins that form Type I intermediate filaments (IFs) in the cytoplasmic cytoskeleton of all mammalian epithelial cells. They are encoded on chromosome 17q and include keratins K9-K10, K12-K28, and K31-K40.