Pan-cadherin Polyklonaler Antikörper

Pan-cadherin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 31313-1-AP

Synonyme

Pan Cadherin, E-cadherin, Arc 1, Cadherin 1, Cadherin-1

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  WB analysis using 31313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 31313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31313-1-AP (Pan-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31313-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31313-1-AP (Pan-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31313-1-AP (Pan-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 31313-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Pan-cadherin antibody (31313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C6-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, MCF-7-Zellen, Maushirngewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

31313-1-AP bindet in WB, IHC, IF/ICC, ELISA Pan-cadherin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: NM_004360
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in tissue morphogenesis and the maintenance of normal tissue architecture (PMID: 23885883). The classic cadherin family members include E-, N-, P-, R-, and B-cadherins. The classic cadherins have ectodomains composed of five extracellular cadherin (EC) repeats, a single transmembrane region, and a cytoplasmic domain. Structural characterization indicated that the known cadherins are glycoproteins ranging in their apparent molecular weights between 120 and 140 kDa (PMID: 2077036). This polyclonal antibody is raised against a synthetic peptide corresponding to a conserved region of human E-cadherin and can detect E-, N-, P-, VE-, and R-cadherins.

Publikationen

Species	Application	Title
human	WB	Cancer Med; circFOXK2 Stabilizes STMN1 mRNA via PABPC1 to Promote the Progression of NSCLC; Authors - Wei Chen
human	WB	Biomaterials; Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy; Authors - Xuemei Yao