Phospho-Tyrosine Rekombinanter Antikörper

Phospho-Tyrosine Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

-

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250495G7

Kat-Nr. : 86398-1-RR

Synonyme

p Tyrosine, Pan-phospho-Tyr, Phospho Tyrosine, Phospho-Tyrosine (P-Tyr-1000), P-Tyr-1000

---

• 

  WB analysis using 86398-1-RR

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 86398-1-RR (Phospho-Tyrosine antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  IF Staining of A431 using 86398-1-RR

  Immunofluorescent analysis of (4% PFA) fixed hEGF6 treated A431 cells using Phospho-Tyrosine antibody (86398-1-RR, Clone: 250495G7 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EGF treated A431 cells
• Erfolgreiche Detektion in IF/ICC: hEGF6 treated A431 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86398-1-RR bindet in WB, IF/ICC, ELISA Phospho-Tyrosine und zeigt Reaktivität mit -

• Getestete Reaktivität: -
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Phospho-Tyrosine
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospho-Tyrosine antibody refers to a class of pan-specific antibodies or detection reagents that recognize all phosphorylated tyrosine residues regardless of the surrounding amino-acid sequence. These tools are widely used to monitor global tyrosine phosphorylation events in signal-transduction studies, cancer research and drug-discovery screens.
This antibody does not cross-react with peptides containing Phospho-Serine or Phospho-Threonine.